Structural requirements of the GDP binding site of elongation factor Tu
نویسندگان
چکیده
منابع مشابه
GTPase center of elongation factor Tu is activated by occupation of the second tRNA binding site.
Interaction of the elongation factor EF-Tu with the antibiotic kirromycin results in activation of the GTPase center of the factor and in induction of an additional tRNA binding site (tRNA binding site II to distinguish it from the classical tRNA binding site I). Activation of the GTPase center under these conditions is stimulated by addition of tRNA. Two-fold evidence is presented that this st...
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15 صفحه اولSequence and Identiflcation of the Nucieotide Binding Site for the Elongation Factor Tu from Thermits Thermophtius Hb8
Two structural genes for the Thermus thermophilus elongation factor Tu (tuf) were identified by cross-hybridization with the tufA gene from E. coli. The sequence of one of these tuf genes, localized on a 6.6 kb Bam HI fragment, was determined and confirmed by partial protein sequencing of an authentic elongation factor Tu from T.thermophilus HB8. Expression of this tuf gene in E. coli minicells...
متن کاملInteraction of a fluorescent analogue of GDP with elongation factor Tu: steady-state and time-resolved fluorescence studies.
A fluorescent derivative of GDP was prepared by the reaction of 2'-amino-2'-deoxy-GDP with fluorescamine. This derivative binds tightly (KD approximately 4.5 X 10(-8) M) to elongation factor Tu (EF-Tu) from Escherichia coli. The emission properties, including spectra, polarizations, and lifetimes, for fluorescamine-GDP free in solution and bound to EF-Tu are presented. Emission data on the fluo...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80095-1